TABLE 1. Dendritic Length and Branching of HEGCs and Normotopic Dentate Granule Cells.
Length (μm) | Branching (# of branch points) | ||
---|---|---|---|
Total (apical + basal) | HEGC - dendrite(s) in ML (N = 34) | 21 13 (761-5225) | 9 (1-18) |
HEGC - dendrite(s) confined to hilus (N = 10) | 1577 (119-3167) | 12 (1-20) | |
GC-SE (N = 20) | 2515 (1053-4584) | 12 (7-22) | |
CGC (N = 17) | 1951 (998-3735) | 10 (4-21) | |
Apical | HEGC - dendrite(s) in ML (N = 34) | 1612 (464-5225)* | 6 (1-17)* |
HEGC - dendrite(s) confined to hilus (N = 10) | 999 (56-2854)** | 6 (1-20) | |
GC-SE (N = 20) | 2458 (1016-4584) | 12 (6-22) | |
CGC (N = 17) | 1879 (731-3735) | 10 (4-21) | |
Basal | HEGC - dendrite(s) in ML (N = 26) | 291 (8-2727) | 2 (0-14) |
HEGC - dendrite(s) confined to hilus (N = 9) | 358 (63-1909) | 2 (0-11) | |
GC-SE (N = 8) | 125 (38-278) | 1 (0-1) |
Values were obtained from Scholl analysis of dentate granule cells labeled by intracellular dialysis with biocytin during whole cell patch clamp re cording. They are expressed as medians and (range) for the number of cells (N) indicated. HEGCs with one or two dendrites in the molecular layer were compared to GC-SEs and CGCs by the Kruskal-Wallis one-way ANOVA on ranks. HEGCs with dendrites confined to the hilus were compared to HEGCs with one or two dendrites in the molecular layer by the Mann-Whitney rank sum test.
P <0.05 compared to GC-SE by Dunn’s test after Kruskal-Wallis one-way ANOVA on ranks yielded P <0.05.
P <0.05 compared to HEGCs with dendrites in the molecular layer by Mann-Whitney rank sum test. CGC, normotopic granule cells from control rats; GC-SE, normotopic granule cells from rats that had experienced status epilepticus; HEGC, hilar ectopic granule cells from rats that had experi enced status epilepticus; ML, molecular layer of the dentate gyrus.