Figure 3.

Immunohistochemical staining for HIVEP3 and SOX9 proteins in PCa and adjacent benign prostate tissues.

Notes: (A) HIVEP3 strongly positive staining was found in the cytoplasm and nucleus of the PCa cell; (B) HIVEP3 weakly positive staining in the PCa was found in the cytoplasm and the nucleus of adjacent benign prostate gland cell; (C) SOX9 strongly positive staining was found in the cytoplasm and the nucleus of the PCa cell; (D) SOX9 moderately positive staining in the PCa was found in the cytoplasm and the nucleus of the adjacent benign prostate gland cell; (E) Negative immunostaining of negative controls with the primary antibody omitted in the PCa tissues; (F) Immunoreactivity scores (IRS) of SOX9 and HIVEP3 in PCa and adjacent benign prostate tissues. Inset areas in A–E is 400X magnification. The arrows in A–D show positive staining cells. **P<0.001 in comparison between PCa and adjacent benign prostate tissues.

Abbreviations: PCa, prostate cancer; IRS, immunoreactivity scores.