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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: Mol Cell Endocrinol. 2013 Dec 1;382(2):989–997. doi: 10.1016/j.mce.2013.11.008

Figure 1. Hypo-glycosylated hFSH isolation from hLH preparations.

Figure 1

FSH bound to anti-hFSHβ immunoaffinity columns was applied to a 10 X 300 mm Superdex 75 column and the chromatogram developed as described under Methods. A. FSHβ and FSHα Western blots of reduced samples from the chromatogram in panel C. Anti-FSHβ: Lane 1, 1 μg hFSH (AFP7298A); lane 2, 1 μg fraction A; lane 3, 1 μg fraction B; lane 4, 1 μg fraction C. Anti-α: Lane 5, 1 μg hFSH (AFP7298); lane 2, 1 μg fraction A; lane 3, 1 μg fraction B; lane 4, 1 μg fraction C. B. FSHβ Western blot of non-reduced samples from the chromatogram in panel C. Lane 1, 1 μg hFSH (AFP7298A); lane 2, 1 μg fraction A; lane 3, 1 μg fraction B; lane 4, 1 μg fraction C. C. First round of hFSH isolation. D. Second round of hFSH isolation. E. Third round of hFSH isolation. The open bars show portions of each chromatogram containing aggregated hFSH (A) hFSH heterodimer (B), and subunits (C).