Figure 1. Identification of trypanosome QS regulators.

a. Selection for genes whose RNAi silencing renders trypanosomes resistant to pCPTcAMP or 8-pCPT-2′-O-Me-5′-AMP, identifying molecules that promote stumpy formation.

b. RNAi libaries were exposed to pCPTcAMP or 8-pCPT-2′-O-Me-5′-AMP, RNAi being induced (1 μg/ml tetracycline), or not. The curves for uninduced samples are combined for clarity (mean ±s.e.m, n=5).

c. Ion Torrent™ read-density from the selected parasites aligned to the trypanosome genome. Since amplicons were fragmented prior to sequencing, reads with and without the flanking primers are shown.