Skip to main content
. Author manuscript; available in PMC: 2014 Jul 30.
Published in final edited form as: Nature. 2013 Dec 15;505(7485):681–685. doi: 10.1038/nature12864

Figure 2. RNAi to the identified genes prevents growth control and morphological transformation.

Figure 2

a. In vivo growth of pleomorphic RNAi lines targeting distinct genes identified from the genome-wide screen for QS-signal resistance. RNAi was induced by provision of doxycyline to the infected animals (n=3, red lines), with parallel infections remaining uninduced (n=3, blue lines). Infections were terminated when the ascending parasitaemias were predicted to become lethal within 12 hours (crosses).

b.Morphology of PP1 RNAi cells and parental T. brucei AnTat1.1 90:13 cells (each grown in mice ±doxycycline) at 6 days post-infection. The induced PP1 cells remained predominently slender in morphology. Cells with slender (SL), intermediate (INT) or stumpy (ST) morphology are labelled. Bar=15μm.

HHS Vulnerability Disclosure