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. Author manuscript; available in PMC: 2014 Jul 30.
Published in final edited form as: Nature. 2013 Dec 15;505(7485):681–685. doi: 10.1038/nature12864

Figure 3. Silencing the identified genes reduces G1 arrest and differentiation competence.

Figure 3

a. Cell-cycle status of pleomorphic RNAi lines. n=6; mean ±s.e.m. percentage 1 kinetoplast (K), 1 nucleus (N) (G0/G1, plus S-phase cells; left Y-axis), 2K1N (G2-phase cells) or 2K2N (post-mitotic cells) (both right Y-axis) are shown. Test genes showed a signficant difference (GLMM, p<0.001) in comparison to AnTat1.1 90:13 +doxycycline on at least one day of infection.

b. PAD1 expression on day 6 post-infection (n=3/group, mean ±s.e.m.). PP1 RNAi cells show reduced PAD1 expression (GLM, F1,4=22.35, p=0.009).

c. PP1-depleted cells show signficantly reduced Procyclin expression during differentiation (GLM, F1,4=10.87, p=0.030) Bars represents mean ±s.e.m; n=3.

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