Table 1. Flow cytometry mix for detection of cytokine produced by lung T cells.
| MIX | POLYCLONAL STIMULATION | EXTRACELLULAR MARKERS | INTRACELLULAR MARKERS |
| Treg MIX | . | anti-CD3 PE-Cy7 | anti-Il10 FITC |
| COMPLETE MEDIUM | anti-CD8 APC-H7 | ||
| Th1 MIX | PHORBOLMYRISTATE-ACETATE (PMA). | anti-CD3 APC | anti-IFNγ FITC |
| anti-CD8 APC-H7 | |||
| Th2 MIX | IONOMYCIN. | anti-CD3 PE-Cy7 | anti-Il4 APC |
| anti-CD8 APC-H7 | anti-Il13 A488 | ||
| BREFELDINE A | anti-Il 5 PE | ||
| COMPLETE MEDIUM. | anti-CD3 PE-Cy7 | ||
| Th17 MIX | PHORBOLMYRISTATE-ACETATE (PMA). | anti-CD8 APC-H7 | anti-Il17 PE |
| IONOMYCIN. | anti-CCR6 A488 | ||
| MONENSIN |