Figure 4. Topographic fluorescence analysis of red beet protoplasts isolated from tissue incubated 24 h in 200 mM sucrose at two different stages. (A) Protoplast from dormant beet hypocotyls with a large central vacuole. (B) Protoplast from tissue incubated in 200 mM sucrose for 24 h. Fluorescence intensity of vacuole-derived vesicles is similar to that emanating from the vacuole.