Table 1. Changes of plasma membrane marker vanadate-sensitive ATPase activity in red beet cells incubated in sucrose or betaine for 24 h.
24 h | ||||
---|---|---|---|---|
Time 0 | Betaine | Sucrose | % increase | |
Vanadate sensitive P-ATPase (*Units/ total ATPase in tonoplast fraction) |
0.17 ± 0.03 | 0.23 ± 0.01 | 0.31 ± 0.02 | 20% |
One unit defined as 1 nkat. Purified tonoplast fractions were isolated from fresh tissue and from tissue incubated in sucrose or betaine for 24 h. V-ATPase activity was measured in the presence and absence of vanadate.