Figure 2. (A) Schematic showing the cellular and molecular changes accompanying adipogenic differentiation. Upon receiving adipogenic signals, C/EBPβ and δ are upregulated leading to induction of C/EBPα and PPARγ2. (B) Induction of adipogenesis-specific transcription factors in hemSCs cultured in adipo media (black bars) for 7 d as compared with cells in control media (white bars) [mRNA normalized to β-actin, * p < 0.05 compared with control media]. (C) TBX2 mRNA levels in cells exposed to adipo media [* p < 0.05 compared with control media]. (D) post-PCR products were run on agarose gel to confirm specific amplification.