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. 2014 Jan 31;9(1):e86636. doi: 10.1371/journal.pone.0086636

Figure 3. NS1619 attenuates PDGF-induced PASMC proliferation.

Figure 3

(A) BK channel opening agent NS1619 (100 µmol/l) reduces PDGF-induced PASMC proliferation (n = 21 each column; * p<0.05). (B,C) In cultured PASMCs, phosphorylation state of protein kinases ERK1, ERK2 and AKT increased significantly 4 minutes after PDGF-BB stimulation (n = 3–6, *:p<0.05). Afterwards, phosphorylation states of ERK1 and ERK2 significantly increased within the period between 4 and 8 minutes after PDGF stimulation (n = 3–6; *:p<0.05) whereas AKT phosphorylation state rose by trend. BK channel opening by NS1619 (100 µmol/l) treatment does not affect phosphorylation of any kinase analysed.