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. 2014 Jan 31;9(1):e87248. doi: 10.1371/journal.pone.0087248

Figure 2. Phenotypic analysis of JKM139-based strains.

Figure 2

(A) Fraction of the colonies that grew after HO endonuclease induction. Deletion mutant for PPH3 or PSY2 had reduced survival and recovered when CHK1 was overexpressed. Overexpression of RAD53 reduced survival when PPH3 or PSY2 were deleted. (B) Fraction of the colonies that grew after HO endonuclease induction when cells were synchronized in G1, S or G2/M phases. (C) Illustration of conceptual basis for the observed activity of Pph3/Psy2 complex and Chk1p in parallel pathways. (D) Illustration of conceptual basis for the observed activity Pph3/Psy2 complex (enzyme) in relationship to Rad53p (substrate). Overexpression of the substrate in the absence of the enzyme can result in a very sick phenotype. [X]↑ refers to overexpression of gene X.