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Single deletion mutants for PPH3 or PSY2 showed increased sensitivity to DSB inducing agent bleomycin. Double deletion mutant strains Δpph3Δchk1 and Δpsy2Δchk1 had elevated sensitivity in comparison to single deletion mutants Δpph3 and Δpsy2. In contrast double deletion mutant strains Δpph3Δrad53 and Δpsy2Δrad53 had reduced sensitivity in comparison to single deletion mutants Δpsy2 and Δpph3.
