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. 2014 Jan 31;9(1):e87226. doi: 10.1371/journal.pone.0087226

Figure 2. Sodium channel NaV1.5 expression in WT and MHC-CnA ventricles.

Figure 2

(A) Protein lysates from four different WT and MHC-CnA ventricles were analyzed for NaV1.5 expression by immunoblotting at weeks (Wk) 0, 1, 2, 3 and 4. (B) Quantification of the blots (ratio of Protein/Ponceau) represented in (A). (C) Representative images from WT and MHC-CnA ventricles immunolabeled for NaV1.5. Scale bar represents 25 µm. (D) Quantification of NaV1.5 labeling intensity exemplified in (C). (E) Scn5a, the gene encoding NaV1.5, was assessed by TaqMan RT-qPCR. MHC-CnA values (B, D and E) are relative to WT (set to 1). Values are mean ± SEM; *p<0.05, **p<0.01 compared to WT.