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. 2013 Apr 16;8(7):e24618. doi: 10.4161/psb.24618

graphic file with name psb-8-e24618-g2.jpg

Figure 2. Transcript levels of CYP71A13, CYP71B15, CYP82F2, MYB51, PR1 and PDF1.2 genes during compatible and incompatible Arabidopsis-P. capsici interactions.Four-to-five-week-old Arabidopsis leaves were plug-inoculated with P. capsici LT123 (gray bars) and LT263 (black bars). Six circular leaf discs (Ø 1 cm), with the inoculation spot in the center, were collected from three plants at the time points indicated on the X-axes (hours post inoculation). Subsequently, total RNA was isolated and used as template for cDNA synthesis. Transcript levels were quantified by real-time PCR using gene-specific primers of which the sequences are shown in the figure and were normalized with ArabidopsisActin 2 (F: 5′-TAACTCTCCCGCTATGTATGTCGC-3′; R: 5′-GAGAGAAACCCTCGTAGATTGGC-3′). Values on the Y-axes are expressed as mean fold changes (± SD) relative to the transcript level in mock-inoculated Col-0 leaves (white bars) at time point 0 which was arbitrary set as 1. Experiments were repeated twice with similar results.