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. 2013 May 7;8(7):e24721. doi: 10.4161/psb.24721

Table 1. Peptide-tandem mass spectrum matches from air (control) and ET-treated A. thaliana etiolated seedlings.

Air treated (control)
Spectrum query EIN2 amino acid start EIN2 amino acid end Observed m/z Mr (expt) Mr (calc) Parent ion ppm error Mascot Ions score Mascot expect value EIN2 peptide sequence match
329 502 506 603.345 602.3378 602.3388 −1.67 25 0.063 D.ESIVR.L
1221474 511 539 1104.5542 3310.6408 3309.6286 306 51 0.00039 R.VKDQLDTTSVTSSVYDLPENILMTDQEIR.S
1221420 511 539 1104.2208 3309.6406 3309.6286 3.63 41 0.0035 R.VKDQLDTTSVTSSVYDLPENILMTDQEIR.S
1221418 511 539 1104.2197 3309.6374 3309.6286 2.64 36 0.0091 R.VKDQLDTTSVTSSVYDLPENILMTDQEIR.S
811749 552 567 886.933 1771.8515 1771.8476 2.23 44 0.00092 K.YSTSQVSSLKEDSDVK.E
812432 552 567 887.4352 1772.8558 1771.8476 569 45 0.00098 K.YSTSQVSSLKEDSDVK.E
811742 552 567 886.9304 1771.8463 1771.8476 −0.73 34 0.006 K.YSTSQVSSLKEDSDVK.E
812434 552 567 887.4361 1772.8576 1771.8476 570 33 0.0073 K.YSTSQVSSLKEDSDVK.E
812425 552 567 887.4332 1772.8519 1771.8476 567 25 0.091 K.YSTSQVSSLKEDSDVK.E
878122 630 647 944.4185 1886.8224 1886.82 1.24 92 0.00000033 K.AAPTSNFTVGSDGPPSFR.S + Phospho S (S)
878123 630 647 944.4192 1886.8238 1886.82 2.01 78 0.0000028 K.AAPTSNFTVGSDGPPSFR.S + Phospho S (S)
878470 630 647 944.9172 1887.8199 1886.82 530 64 0.000017 K.AAPTSNFTVGSDGPPSFR.S + Phospho S (S)
878121 630 647 944.4182 1886.8219 1886.82 0.98 56 0.000034 K.AAPTSNFTVGSDGPPSFR.S + Phospho S (S)
878126 630 647 944.4207 1886.8269 1886.82 3.63 62 0.00005 K.AAPTSNFTVGSDGPPSFR.S + Phospho S (S)
878117 630 647 944.4091 1886.8036 1886.82 −8.72 58 0.000087 K.AAPTSNFTVGSDGPPSFR.S + Phospho S (S)
878472 630 647 630.2815 1887.8227 1886.82 531 26 0.096 K.AAPTSNFTVGSDGPPSFR.S + Phospho S (S)
936929 648 666 668.6009 2002.7807 2001.7847 498 33 0.06 R.SLSGEGGSGTGSLSRLQGL.G + 2 Phospho S (S); Phospho T (T)
423230 700 710 646.8458 1291.6771 1291.6772 −0.094 44 0.0026 K.KLDQLFGTDQK.S
423193 700 710 646.8409 1291.6673 1291.6772 −7.65 30 0.075 K.KLDQLFGTDQK.S
542771 754 766 703.8643 1405.714 1405.7201 −4.39 69 0.000033 R.TPGSIDSLYGLQR.G
542775 754 766 703.8674 1405.7203 1405.7201 0.12 59 0.000064 R.TPGSIDSLYGLQR.G
542786 754 766 703.8703 1405.726 1405.7201 4.2 50 0.00038 R.TPGSIDSLYGLQR.G
542779 754 766 703.8681 1405.7217 1405.7201 1.13 44 0.0011 R.TPGSIDSLYGLQR.G
608871 754 766 743.8513 1485.6881 1485.6865 1.09 31 0.04 R.TPGSIDSLYGLQR.G + Phospho S (S)
608879 754 766 743.8526 1485.6907 1485.6865 2.87 35 0.068 R.TPGSIDSLYGLQR.G + Phospho S (S)
274965 835 843 551.3035 1100.5925 1100.5938 −1.16 45 0.0099 K.ERLEALQSR.G
754564 922 936 841.355 1680.6954 1680.6888 3.91 31 0.0072 K.YSSMPDISGLSMSAR.N + Phospho S (S)
52751 1211 1217 699.45 698.4427 698.4439 −1.65 20 0.062 A.AKPAKGK.C
790762 1269 1284 868.8923 1735.77 1735.7713 −0.74 31 0.062 R.LSNKPVGMNQDGPGSR.K + Phospho S (S)
Ethylene treated
Spectrum query EIN2 amino acid start EIN2 amino acid end Observed m/z Mr (expt) Mr (calc) Parent ion ppm error Mascot Ions score Mascot expect value EIN2 peptide sequence match
902435 511 539 1104.2163 3309.6271 3309.6286 −0.46 44 0.0012 R.VKDQLDTTSVTSSVYDLPENILMTDQEIR.S
902516 511 539 1104.5494 3310.6265 3309.6286 302 34 0.016 R.VKDQLDTTSVTSSVYDLPENILMTDQEIR.S
902436 511 539 1104.2182 3309.6327 3309.6286 1.24 29 0.056 R.VKDQLDTTSVTSSVYDLPENILMTDQEIR.S
902520 511 539 1104.5546 3310.6419 3309.6286 306 31 0.087 R.VKDQLDTTSVTSSVYDLPENILMTDQEIR.S
518666 552 567 591.6218 1771.8437 1771.8476 −2.22 26 0.06 K.YSTSQVSSLKEDSDVK.E
536908 630 647 904.4321 1806.8497 1806.8537 −2.2 55 0.00013 K.AAPTSNFTVGSDGPPSFR.S
241596 700 710 646.8446 1291.6747 1291.6772 −1.98 59 0.00026 K.KLDQLFGTDQK.S
242470 700 710 647.3521 1292.6897 1291.6772 784 35 0.013 K.KLDQLFGTDQK.S
241600 700 710 646.8446 1291.6747 1291.6772 −1.93 32 0.04 K.KLDQLFGTDQK.S
778132 805 826 819.3732 2455.0977 2454.1022 406 65 0.000017 R.APSSSEGWEHQQPATVHGYQMK.S
778133 805 826 819.3735 2455.0987 2454.1022 406 71 0.000056 R.APSSSEGWEHQQPATVHGYQMK.S
777982 805 826 819.0399 2454.0977 2454.1022 −1.84 39 0.0054 R.APSSSEGWEHQQPATVHGYQMK.S
142268 835 843 551.3033 1100.592 1100.5938 −1.61 37 0.065 K.ERLEALQSR.G
309469 839 850 698.3436 1394.6727 1393.6715 718 23 0.096 E.ALQSRGEIPTSR.S + Phospho S (S)
461623 1269 1284 828.9051 1655.7956 1655.8049 −5.63 58 0.000044 R.LSNKPVGMNQDGPGSR.K
461625 1269 1284 828.9077 1655.8009 1655.8049 −2.46 38 0.0029 R.LSNKPVGMNQDGPGSR.K
461621 1269 1284 828.9038 1655.7931 1655.8049 −7.18 37 0.0054 R.LSNKPVGMNQDGPGSR.K
461619 1269 1284 552.9373 1655.7901 1655.8049 −8.95 31 0.051 R.LSNKPVGMNQDGPGSR.K

Spectra from Chen et al.6 were searched as described in Chen et a.l,6 but for semi-tryptic termini. Only the peptide-spectrum matches with expect values < 0.1 are considered.