Table 1. Primers used in this study.
|
promoter cloning, At5g28520 |
Pro5g28520_F | ACCAAGCTTAGAGCTTCAAATCATT |
|---|---|---|
| Pro5g28520_R | AACGGATCCCACGAACTGTTTTGA | |
| Real-time PCR | Pre846_F | ACGATTGGAAGCTGAATGGTTGCG |
| Pre846_R | CGAATTTACCGGGATACACGAGCA | |
| Pre842_F | TAAGCGGGATGATGAATCCGACCA | |
| Pre842_R | TGATGGTGTTTCGATCCCTGGACA | |
| At5g28520_QRT_F | GTCGTGAAGGCATTCAGTACGTCA | |
| At5g28520_QRT_R | TTGTGTGAATCCTCCTCTACCCGA | |
| 18S_F | AAATACCGCGGCTGCTGGCA | |
| 18S_R | CGGCTACCACATCCAAGGAA | |
| ACTIN8_F | GGAATGGTTAAGGCTGGATTCG | |
| ACTIN8_R | ACGCATCTTTCTGATTCATCCC | |
| 5′ RACE | 5RACE1 | CGAATTTACCGGGATACACGAGCA |
| 5RACE2 | CCTTGAATGAAACCGGATGTGGCA | |
| 5RACE3 | CGAGGCGGTTAATCTCAAACTGCT | |
| 5RACE4 | TGATGGTGTTTCGATCCCTGGACA | |
| Northern | At5g28520CDS_F | ATGGCTCAGAAGTTAGAAGCAATAG |
| At5g28520CDS_R | TCACTCGCGCGTGATGGGC | |
| 18S primer pair | same as 18S_F and 18S_R pair used in real-time PCR | |
| Southern | BASTA_F | GGTCTGCACCATCGTCAACC |
| BASTA_R | CCAGAAACCCACGTCATGCC |