Figure 1. RNA gel blot analyses showing diurnal rhythm of OsSOS genes in shoots of IR64 rice seedlings. Seedlings were grown under the 12 h light/12 h dark cycle for 14 d. Shoot samples were harvested at 3 h intervals for 2.5 d under the 12 h light/12 h dark. Twenty µg RNA samples was used for northern blot hybridization. Ethidium bromide (EtBr) stained RNA-gel has been shown as the loading control. Y-axis shows relative transcript abundance of genes while numbers on X-axis shows different time points. Shaded area indicates dark period and white area indicates light period. (A) OsTOC1 shows rhythmic expression and has a peak during light to dark transition. (B) OsSOS3 expression peaks during night. (C) mRNA level of OsSOS2 show rhythmicity in 24 h cycles and show higher expression during the transition period of light to dark. (D) Transcript level of OsSOS1 doesn't show any clear rhythmicity.