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. 2014 Jan 31;9(1):e87546. doi: 10.1371/journal.pone.0087546

Figure 7. Knockdown of sox2 results in abnormal parapineal development and disruption of the habenular asymmetries.

Figure 7

(A) gfi1ab is expressed in parapineal cells on the left side of the brain in control embryos. (B–D) sox2 morphants are categorized into three groups according to gfi1ab expression: left-sided expression (B), right-sided (C) and embryos with scattered gfi1ab cells (D). (E) kctd12.1 is asymmetrically expressed in the habenulae, with a broader expression domain in the left than the right habenula. (F–H) In sox2 morphants kctd12.1 expression can be: asymmetric with more on the left side similar to control embryos (F), asymmetric with more on the right side (G) or symmetric (H). (I) A table showing the percentage of embryos with normal, reversed or bilateral parapineal organs, using different staining methods. Scale bars = 25 µm. See also Figure S9–S10 and Movie S4.