Table 1. Suboptimal doses of sox2 morpholinos result in increased number of PhRs and abnormal positioning of the parapineal organ.
| Number of PhRs a | Parapineal position b | |||
| Left | Right | Bilateral | ||
| Control | 18 (±1.12, n = 16) | 100% (n = 34) | 0% (n = 0) | 0% (n = 0) |
| sox2MO1+sox2MO2 (0.9 ng of each) | 29 (±1.25, n = 16) | 82% (n = 27) | 15% (n = 5) | 3% (n = 1) |
| sox2MO1+sox2MO2 (1.8 ng of each) | 36 (±2.3, n = 13) | 64% (n = 29) | 4% (n = 2) | 31% (n = 14) |
a
Average number of PhRs (± standard error, number of embryos analyzed).
b
Percentage of embryos with left, right or bilateral parapineal projections (number of embryos in each category).