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. 2014 Jan 31;9(1):e87587. doi: 10.1371/journal.pone.0087587

Figure 4. MuRF1 and atrogin1 mRNA and protein expression in WT ALI mice.

Figure 4

MuRF1 (A) and atrogin1 (B) mRNA normalized to GAPDH in the PCA, CT and EDL of SHAM and ALI mice reveal upregulation of atrogin1 in all muscles of ALI mice, but upregulation of MuRF1 only in the CT and EDL, not the PCA of ALI mice. Muscle lysates probed for MuRF1 protein, normalized to GAPDH (C), and quantified by densitometry (D) reveal reduced basal expression of MuRF1 in the PCA of SHAM mice and upregulation of MuRF1 protein in the CT and EDL, but not the PCA of ALI mice. *p<0.05, **p<0.01; n = 3/group.