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. 2014 Jan 31;9(1):e87563. doi: 10.1371/journal.pone.0087563

Figure 3. Effects of miR-222-3p on cell cycle, invasion ability and secretion of MMPs.

Figure 3

(A) MiR-222m-transfected RL95-2 cells and miR-222i-transfected AN3CA cells were subjected to fluorescence-activated cell sorting analysis, and the relative G1, S, and G2/M compartments calculated. Data represent the average percentage of cells in each compartment in three independent experiments, each performed in triplicate. (B) Representative photos and statistical plots of transwell assays in RL95-2 cells transfected with miR-222m and miR-222m NC, and AN3CA cells transfected with miR-222i and miR-222i NC (×100). More cells traversed the transwell membrane in miR-222m transfected cells, and fewer did so in the miR-222i transfected AN3CA cell line. To exclude the effect of cell proliferation, relative cell invasion ability of RL95-2 and AN3CA cells was analyzed by normalizing relevant invasive cell numbers to 48 h MTT OD values. The results in both cells showed that high level of miR-222-3p promoted the ability of relative cell invasion. (C) MMP-2 and MMP-9 decreased significantly in cells transfected with miR-222m and increased in miR-222i transfected cells. Data are expressed as mean±SD. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001.