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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Cell Rep. 2012 Dec 20;2(6):1513–1520. doi: 10.1016/j.celrep.2012.10.011

Figure 3. Lsd1/2 amine-oxidase activities are required for replication fork pausing at MPS1 and imprinting.

Figure 3

(A) Analysis of the DSB at mat1. The genomic DNA is analyzed by Southern blot, as in Figure 2. The relevant genotypes of the strains, the sizes and identities of the DNA fragments are indicated.

(B) MPS1 activity in demethylase mutants. 2D gel analysis of the replication fork intermediates at mat1, showing that the accumulating replication material at the apex of the Y-arc is not significantly reduced in single lsd1-ao and lsd2-ao mutants but is diminished in the double mutant strain. The gels are treated as in Figure 2 and the relevant genotypes are indicated. The percentage of pause is indicated for each strain from 2 independent 2D gels.