Table 1. Normalized luminosity indices of Nkcc/Ncc and Nka immunostaining in the gills, anterior intestine (AI) and posterior intestine (PI) of Mozambique tilapia.
| Tissues and Conditions (number of replicates) | Normalized Luminosity Index* for Nkcc/Ncc | Normalized Luminosity Index* for Nka |
| Gill | ||
| Freshwater (FW; n = 4) | 0.811±0.016a | 0.726±0.013a |
| Seawater (SW; n = 3) | 0.922±0.010b | 0.824±0.010b |
| Hypersaline water (HSW; n = 3) | 0.996±0.024c | 0.871±0.033b |
| P-value ** | <0.001 | <0.005 |
| Anterior Intestine (AI) | ||
| Freshwater (FW; n = 4) | 0.803+0.037a | 0.807+0.033a |
| Seawater (SW; n = 3) | 0.975+0.063b | 0.908+0.049a |
| Hypersaline water (HSW; n = 3) | 1.013+0.056b | 1.006+0.066a |
| P-value | <0.05 | 0.061 |
| Posterior Intestine (PI) | ||
| Freshwater (FW; n = 4) | 0.853+0.014a | 0.842+0.018a |
| Seawater (SW; n = 3) | 0.956+0.075ab | 0.918+0.068a |
| Hypersaline water (HSW; n = 3) | 1.074+0.055b | 0.987+0.053a |
| P-value | <0.05 | >0.1 |
*
Normalized Luminosity Index (mean±SEM) is expressed as a ratio of the luminosity of Alexa 488 (Nka) or Alexa 568 (Nkcc/Ncc) to DAPI (nuclei) staining within total field (0.307 mm2); the DAPI nuclear staining is used to correct for differences between fields.
**
The data were analyzed by one-way ANOVA followed by post-hoc Duncan's multiple range test (P<0.05 is considered significant). Different alphabets indicate statistical significance from each other.