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. 2014 Jan 31;9(1):e87862. doi: 10.1371/journal.pone.0087862

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© 2014 Patel et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Histogram reporting gene promoter activity of E. coli harboring pMULTIAHLPROM in the presence of either pBBR, pBBR-psaR1 and pBBR-psaR3 plasmids and different hydroxy AHLs (OH-C6-AHL, OH-C8-AHL, OH-C10-AHL and OH-C12-AHL). All measures were performed in biological triplicates, and the mean Miller units with standard deviations are shown. Promoter activity was statistically significantly (P<0.05) increased (a) in the presence of OH-C6-AHL and OH-C8-AHL for PsaR1 compared to respective pBBR empty vector control. Similarly, for PsaR3 statistically significantly (P<0.05) increase (a) in lacZ expression was observed in the presence of OH-C6-AHL, OH-C8-AHL, OH-C10-AHL and OH-C12-AHL compared to respective pBBR empty vector controls.