. 2014 Jan 31;9(1):e87862. doi: 10.1371/journal.pone.0087862

Table 1. Bacterial strains used in this study.

Strains	Relevant characteristics^a	Reference/Source
*E. coli*
DH₅a	Cloning strain, Nal^r	[72]
PRK2013	Helper strain for tri-parental conjugation, Km^r	[77]
E. coli (pSB401)	Biosensor strain; Tc^r	[70], [73]
*Biosensors*
A. tumefaciens NT1	Harbouring pZLR4 plasmid, β-galactosidase reporter system, Gm^r	[95]
C. violaceum CV026	Violacin pigment reporter system, Km^r	[79]
*Pseudomonas syringae* pv. *actinidiae* ( *Psa* )
Psa 10,22	Wild type; Italian isolate; Nf^r	Lab collection
Psa 10,24	Wild type; Italian isolate; Nf^r	Lab collection
Psa 10,25	Wild type; Italian isolate; Nf^r	Lab collection
Psa 10,29	Wild type; Italian isolate; Nf^r	Lab collection
Psa 10,30	Wild type; Italian isolate; Nf^r	Lab collection
Psa 11,41	Wild type; Italian isolate; Nf^r	Lab collection
Psa 11,47	Wild type; Italian isolate; Nf^r	Lab collection
Psa 11,50	Wild type; Italian isolate; Nf^r	Lab collection
Psa 11,51	Wild type; Italian isolate; Nf^r	Lab collection
Psa 12,56	Wild type; Italian isolate; Nf^r	Lab collection
Psa 12,64	Wild type; Italian isolate; Nf^r	Lab collection
Psa-mR1	psaR1::pKNOCK; Nf^r, Km^r; derivative of wild type	This work
Psa-mR2	psaR2::pKNOCK; Nf^r, Km^r; derivative of wild type	This work
Psa-mR3	psaR3:: in-frame deletion mutant generated by pEX19Gm plasmid; Nf^r;derivative of wild type	This work
Psa-mR1+pBBR-psaR1	Psa-mR1 carrying full length psaR1 in pBBR; Nf^r, Km^r, Gm^r; derivative of Psa-mR1	This work
Psa-mR2+pBBR-psaR2	Psa-mR2 carrying full length psaR2 in pBBR; Nf^r, Km^r, Gm^r; derivative of Psa-mR2	This work
Psa-mR3+pBBR-psaR3	Psa-mR3 carrying full length psaR3 in pBBR; Nf^r, Gm^r, derivative of Psa-mR3	This work
Psa-mR3+pcos-psaR3	Psa-mR3 carrying cosmid clone for psaR3; Nf^r, Tc^r, derivative of Psa-mR3	This work
Psa-mR3+ Psa-mR1	Psa-mR3 and Psa-mR1 double mutant; Nf^r, Km^r; derivative of Psa-mR3	This work
Psa-mR3+ Psa-mR2	Psa-mR3 and Psa-mR2 double mutant; Nf^r, Km^r; derivative of Psa-mR3	This work
Psa-mR3+ Psa-mR1+ pcos-psaR3+pBBR-psaR1	Psa-mR3 and Psa-mR1 double mutant carrying cosmid clone for psaR3 and fulllength psaR1 in pBBR; Nf^r, Km^r, Tc^r, Gm^r; derivative of Psa-mR3+ Psa-mR1	This work
Psa-mR3+ Psa-mR2+ pcos-psaR3+pBBR-psaR2	Psa-mR3 and Psa-mR2 double mutant carrying cosmid clone for psaR3 and fulllength psaR2 in pBBR; Nf^r, Km^r, Tc^r, Gm^r; derivative of Psa-mR3+ Psa-mR2	This work

Nal^r, Km^r, Tc^r, Gm^r, and Nf^r indicate resistance to nalidixic acid, kanamycin, tetracycline, gentamycin and nitrofurantoin respectively.