Table 5. Swarming and swimming movement score of Pseudomonas syringae pv. actinidiae strains.
Psa Strains | Swarming (0.8% KBA)Average ± S.D. in mm | Swarming (0.6% KBA)Average ± S.D. in mm | Swimming 0.3% KBA)Average ± S.D. in mm |
Psa (WT) | 65.33±1.5 | 80.00±0.5 | 66.50+/−5.8 |
Psa-mR1 | 64.00±1.48 | 75.00±3.13 | 65.00±5.22 |
Psa-mR2 | 63.00±1.04a | 77.50±2.71 | 65.50±3.73 |
Psa-mR3 | 55.00±1.04a | 72.00±2.09a | 37.50±7.83a |
Psa-mR1+pBBR-psaR1 | 54.33±0.49a | 79.00±1.04 | 62.00±2.95 |
Psa-mR2+pBBR-psaR2 | 50.00±1.20a | 77.00±2.33 | 59.00±1.04 |
Psa-mR3+pBBR-psaR3 | 37.00±0.60a | 72.50±2.71a | 37.50±7.96a |
Psa-mR3+Psa-mR1 | 30.25±0.62a | 69.50±3.92a | 33.00±3.13a |
Psa-mR3+Psa-mR2 | 30.58±0.80a | 65.50±1.50a | 27.00±3.14a |
Psa-mR3+Psa-mR1+ pcos-psaR3+ pBBR-psaR1 | 7.50±0.522a | 10.50±0.90a | 29.00±1.04a |
Psa-mR3+Psa-mR2+ pcos-psaR3+ pBBR-psaR2 | 21.00±1.04a | 57.50±2.61a | 40.00±4.35a |
Mean values and standard deviations were calculated for swarming and swimming bacterial movement obtained from three replications each on 0.8%, 0.6% and 0.3% KBA. Statistical analyses (Student’s t test) were performed to compare the significant difference in bacterial movement between wild type Psa strain and mutated and complemented strains. a, significant difference to WT at P<0.0001.