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. 2014 Jan 31;9(1):e87767. doi: 10.1371/journal.pone.0087767

Figure 3. Validation of IL-8 as a target of miRNA-23a.

Figure 3

(A) (top) Diagrammatic representation of binding site of miRNA-23a in the 3′UTR of IL-8; (bottom) miRNA-23a mimic significantly reduced the luciferase activity of a dual luciferase reporter with the 3′UTR of IL-8 compared to the controls. Values are the means ± SD of percent changes over controls after normalization to the Renilla luciferase activity. (B) A representative result of Western blot shows the expression level of IL-8 in the CNE2 and CNE2-IR cells, and CNE2-IR cells transfected with miRNA-23a mimic or mimic control. β-actin was used as an internal control for loading. Three experiments were done; columns, mean; bars, S.D. *P<0.05 and **P<0.01 differ from the controls. DLR-blank, a dual luciferase reporter without the 3′UTR of IL-8; DLR-IL8 3′UTR, a dual luciferase reporter with the 3′UTR of IL-8.