Skip to main content
. 2014 Jan 31;9(1):e87767. doi: 10.1371/journal.pone.0087767

Figure 5. The roles of miRNA-23a and IL-8 in the radioresistance of NPC cells.

Figure 5

(A) and (B). A representative clonogenic survival assay shows that transfection of miRNA-23a mimic decreased the radioresistance of NPC CNE2-IR cells. CNE2-IR cells and its transfectants were irradiated with a range of 2-10 Gy radiation doses, and colonies that formed after incubation of 12 d were counted to calculate the survival fractions, and dose survival curve was drawn. (C) Hoechst 33258 staining shows that transfection of miRNA-23a mimic increased the apoptosis of irradiation-induced CNE2-IR cells. CNE2-IR cells and its transfectants were exposed to 6 Gy irradiation, incubated for 48 h, and then assessed for cell apoptosis using the cell-permeable DNA dye Hoechst 33258. (D) A histogram shows the apoptotic rate of CNE2-IR cells and its transfectants 48 h after 6 Gy irradiation. (E) and (F) A representative clonogenic survival assay shows that neutralization of secretory IL-8 using anti-human IL-8 antibody decreased the radioresistance of NPC CNE2-IR cells. CNE2-IR cells were cultured with DMEM medium supplemented with 2% FCS and monoclonal mouse anti-human IL-8 antibody (2.5 µg/mL) or mouse control IgG1 (2.5 µg/mL), and irradiated with a range of 2-10 Gy radiation doses, and colonies that formed after incubation of 12 d were counted to calculate the survival fractions, and dose survival curve was drawn.