Figure 2. Principal component analysis (PCA) loadings plots of ¹H NMR spectral data.

DLLME extracts were prepared from cells treated with 0 (control), 50, 150, 300 and 1000 nM ergosterol, and incubated for 18 h. The loadings plot shows the discriminating variables responsible for sample clustering depicted by the scores plots in Fig. 1 (B and C). (A) PC1 vs. PC2 and (B) PC1 vs. PC3.