Figure 8. The quantitative, time-dependent, responses of some of the annotated compounds from different metabolite classes:

(A–C) sesquiterpenoid phytoalexins (rishitin, solavetivone and phytuberin), (D–E) sterols (squalene and stigmasterol) and (F–H) phenolics (shikimate, caffeoyl shikimate and coniferyl alcohol). This relative quantification is based on their respective peak areas estimated from peak integration. The quantitative estimation shows a time-dependent biosynthesis of sesquiterpenoids, sterols and phenylpropanoids from cells treated with 300 nM ergosterol, reaching a maximum at 18 h post-treatment (N-ANOVA t-test, p<0.0001).