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. 2014 Jan 31;9(1):e87794. doi: 10.1371/journal.pone.0087794

Figure 4. Function of T-cell subsets in IFN-OFF patients.

Figure 4

A) T-cells were stimulated with anti-CD3, –CD28 and -CD49d and after 6 h incubation, cell surface markers and intracellular cytokines were stained and analyzed with flow cytometry. The cytokine secretion of CD4+ T-cell subsets (effector and central memory, naïve and temra cells) was analyzed separately. The percentage values present the proportion of cytokine secreting cells from all CD4+ cells showing that the effector and central memory T-cells are the main cytokine producers. B) The proportion of cytokine secreting effector and central memory CD4+ T-cells were also analyzed separately and the % values show the proportion of positive cells from a subset in question. C) Cytokine secretion of CD8+ T-cell subsets and D) the proportion of cytokine secreting effector and central memory CD8+ T-cells separately. E) Degranulation of CD8+ T-cells was analyzed by CD107a/b expression with flow cytometry after 6 h incubation with anti-CD3, -CD28 and -CD49d. Unstimulated MNCs were used as a control.