Figure 3. Effect of antibiotics on intracellular growth of L. pneumophila during an infection and in a ‘phagocytic plaque assay’.

A. Left, representative experiment from a series with similar outcome of intracellular growth kinetic of GFP-expressing L. pneumophila measured by fluorescence intensity in the presence of 30 µM of antibiotics. Right, normalized intracellular bacterial growth (DMSO = 1) in A. castellanii, error bars represent the standard deviation from biological triplicates. B. Ability of D. discoideum DH1 strain (1000 cells/well) to grow on a bacterial lawn composed of M. marinum and Klebsiella pneumoniae (2::1 ration) after seven days and in presence of antibiotic compounds.