Figure 5. Secondary assays for identifying compounds properties.
A. Basic scheme of screen workflow including the growth inhibition/cytotoxicity assay and the antibiotic assay before hit validation in other host models system. B. Growth inhibition/cytotoxicity assay. Growth kinetics of GFP-expressing D. discoideum AX2 cells measured by fluorescence intensity, in the presence of various concentrations of ethambutol (top) or nicotin (bottom). C. antibiotic assay. Left, intracellular and Right, intracellular growth kinetic of GFP-expressing M. marinum measured by fluorescence intensity in the presence of various concentrations of fusidic acid, accompanied by the corresponding graphs for IC50 determination. B and C are representative experiments from a series with similar outcome.