Table 1. Inhibitory concentrations 50% (IC₅₀) values for M. marinum growth in extracellular and intracellular conditions.

antibiotic	extracellular IC50 (µM)	intracellular IC50 (µM)
streptomycin	0.1–1	3–7
fusidic acid	0.01–0.1	3–5
ethionamide	0.1–1	3–10
isoniazid	5–10	20–30
rifampin	0.1–0.5	3–10
rifabutin	2–3	1–3
ethambutol	5–10	5–10
levofloxacin	10–30	>30
moxifloxacin	5–10	>30
thioridazine	1–5	5–10
PA-824	3–5	5–10

As presented in Figure 5C, GFP-expressing M. marinum growth kinetics were measured in broth culture and during cellular infection in presence of antibiotics in a concentration range, and IC₅₀ values were graphically determined from a series of experiments with similar outcome.