Table 3. Characteristics of 13 polymorphic cSSRs loci in H. chinensis.
| Locus | GeneBank accession | Primer sequence | Tm(°C) | Repeat type | Size range/bp | N | Ne | HWE (P) | PIC | Null | Ho/He |
| comp30707_c0_seq1 | KC528797 | F:TATTCCTTTATTTAATGCTGTC | 50 | (CT)6 | 160–164 | 3 | 2.65 | 0.07 | 0.55 | 0 | 0.53/0.63 |
| R:GAGACGGATTCCCTTGAGTA | |||||||||||
| comp5875_c0_seq1 | KC528798 | F:TCATACACGGATTCATACAGA | 58 | (TA)7 | 121–125 | 3 | 2.99 | 0.33 | 0.59 | 0 | 0.63/0.66 |
| R:TGGAATAGACTTACGAATAAGAG | |||||||||||
| comp77539_c0_seq1 | KC528799 | F: AGCACTAAACCCATCCCAAT | 58 | (AC)9 | 100–106 | 4 | 3.49 | 0.39 | 0.66 | 0 | 0.60/0.72 |
| R: GCAGAGTCTGATGGGTGTATA | |||||||||||
| comp5228_c0_seq3 | KC528800 | F: ATCCAAGTTTGCCGTCAG | 58 | (AC)16 | 98–106 | 3 | 2.96 | 0.66 | 0.59 | 0 | 0.69/0.67 |
| R: AATCCGCCTCCTCGCTCT | |||||||||||
| comp7499_c0_seq1 | KC528801 | F: TCCTGTTAGTCTGAAAAGCC | 58 | (GA)8 | 143–153 | 6 | 4.98 | 0.35 | 0.77 | 0 | 0.79/0.81 |
| R: TCTCACGTAATGATTCTCCA | |||||||||||
| comp16428_c0_seq1 | KC528802 | F: ATGTCTATTGAGTGACTTGCT | 58 | (TG)8 | 139–143 | 3 | 2.97 | 0.01 | 0.59 | 1 | 0.41/0.67 |
| R: ATTGTAATGGTTTGGAGGAT | |||||||||||
| comp35785_c0_seq1 | KC528803 | F: TGTGACAGATGAGAATGAAACT | 55 | (TG)8 | 169–177 | 3 | 2.92 | 0.09 | 0.58 | 0 | 0.50/0.67 |
| R: TAGGGAAGGACAAAGCCA | |||||||||||
| comp11916_c0_seq1 | KC528794 | F: GCACTAGGGCACCCATAA | 58 | (CT)10 | 100–104 | 3 | 2.60 | 0.06 | 0.54 | 0 | 0.73/0.63 |
| R: TTTACGCTCTTTCCCCAA | |||||||||||
| comp15080_c0_seq5 | KC528805 | F: AGATGACAGAGGGGAGATTT | 58 | (GT)10 | 159–169 | 4 | 2.97 | 0.64 | 0.61 | 0 | 0.77/0.67 |
| R: GGCGGTGAGGAGGATGAGCG | |||||||||||
| comp8900_c1_seq1 | KC528806 | F: TCAATGGCATTATCAGTCC | 56 | (TA)12 | 143–147 | 3 | 2.87 | 0.81 | 0.58 | 0 | 0.63/0.66 |
| R: CCCCTTTTACACGGTCAC | |||||||||||
| comp55574_c0_seq1 | KC528807 | F: TACCTGGCTCTGTCCCT | 58 | (AC)10 | 238–244 | 4 | 3.92 | 0.01 | 0.70 | 1 | 0.50/0.76 |
| R: AACCTGCCTCTTTCCC | |||||||||||
| comp38566_c0_seq1 | KC528808 | F: GGAAAATCGCAGACACG | 58 | (AG)8 | 237–247 | 5 | 4.04 | 0.22 | 0.71 | 0 | 0.90/0.77 |
| R: CACATAGGAAATGACTCGG | |||||||||||
| comp32386_c0_seq1 | KC528809 | F: AAGCATTCCAGAAGGCG | 58 | (TG)7 | 234–244 | 6 | 4.88 | 0.00* | 0.77 | 1 | 0.53/0.81 |
| R: AACGGACCAGGGATACA | |||||||||||
| Average | 3.85 | 3.4 | 0.63 | 0.63/0.70 | |||||||
N, number of alleles; Ne, effective number of alleles; He/Ho, expected and observed heterozyosity; HWE, Hardy-Weinberg equilibrium; “*” show significant deviation from Hardy-Weinberg equilibrium (p<0.0026).