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. 2013 Dec 24;2:73–81. doi: 10.1016/j.redox.2013.12.012

Fig. 5.

Fig. 5

Analysis of colonic mucus thickness and goblet cell abundance with nitrite supplementation. (A) Firmly adherent mucus thickness (μm) in untreated mice (n=6), DSS-treated mice (n=5) and mice with DSS+nitrite supplementation (n=5). (B) Representative pictures of histological colon samples stained with Periodic Acid Schiff/Alcian Blue (PAS/AB) (scale bar 50 µm) and (C) quantification of the number of goblet cells in colon samples of untreated mice, DSS-treated mice and mice with DSS+nitrite supplementation (n=8 in each individual group). (D) Quantification (a.u.) of colonic Muc2 expression by immunohistochemistry staining (n=10 in each individual group) (P<0.05, ⁎ vs. control, # vs. DSS-group).