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. 2014 Jan 31;9(1):e87918. doi: 10.1371/journal.pone.0087918

Figure 6. Analysis of global methylation in MEFs, HCC and osteosarcoma cells and DNMTs levels in MEFs.

Figure 6

An ELISA-based global methylation assay was performed to determine changes in global methylation levels due to oxidative stress as a result of hypoxia and ROS exposure. The histograms are representative of three independent experiments and the error bars depict the +/− SD. (a) In MEFs the values have been normalized to the untreated wild-type cells. (b,c) The values have been normalized to the respective untreated samples. (d) Western blot analysis was used to determine the levels of the DNMTs from whole cell lysates extracted from untreated (−) and treated (+) MEF cells.