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. 2014 Jan 15;4(1):130090. doi: 10.1098/rsob.130090

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© 2014 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 2. — BR_187–385 is a monomer in solution. (a) The continuous distribution of sedimentation coefficients reveals a single population for the BR_187–385 monomer with a sedimentation coefficient of 1.85 S. The averaged theoretical sedimentation coefficients calculated for the SAXS-derived BR_187–385 monomer models are in agreement with the experimentally determined value. The averaged value and the standard deviation are in red and dotted red, respectively. (b) The theoretical scattering curve obtained for an ensemble of 18 BR_187–385 monomer models (see also electronic supplementary material, figure S1) fits the SAXS profile of BR_187–385 with a χ²-value of 0.58. The radius of gyration R_g obtained from the Guinier approximation was 22.7 ± 1.2 Å (mean ± s.d.). The distance distribution function p(r) gave R_g and D_max values of 22.5 ± 2.0 Å and 77.0 ± 8.0 Å, respectively.