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. 2014 Jan 22;4(1):130202. doi: 10.1098/rsob.130202

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© 2014 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 1. — Binding of C. jejuni to BgAgs and identification of C. jejuni BgAg-binding adhesins. (a) Binding of digoxigenin (DIG)-labelled C. jejuni NCTC11168 to ELISA wells coated with BgAgs. Specific binding was calculated by subtracting the bovine serum albumin (BSA; negative control) values from the BSA–BgAg absorbance. Binding was significantly inhibited (p < 0.05) by the pre-incubation of bacteria with soluble glycoconjugates H-II, Le^b or Le^y prior to adding to the ELISA plate. NT, non-treated. Error bars: mean of triplicate values ± s.e.m. from three independent experiments. (b) Identification of BgAg-binding proteins from C. jejuni NCTC11168 using re-tagging (baits used: H-II; H-I and Le^b). Two proteins were identified at sizes of 45 and 59 kDa, corresponding to MOMP and FlaA, respectively.