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. 2014 Jan 22;14:7. doi: 10.1186/1472-6823-14-7

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Copyright © 2014 Svensson et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Release of adiponectin and omentin from human adipose tissue. Evaluation of the final incubation protocol including HSA. (A) Subcutaneous adipose tissue (SAT) from 10 subjects (5 obese and 5 nonobese) and (B) 11 paired biopsies of subcutaneous and visceral adipose tissue (VAT) were incubated for 2, 4, 6, 8, and 24 h in medium containing 1% HSA according to our final incubation protocol. The media were analyzed for (A) adiponectin and (B) omentin by ELISA. As expected, adiponectin was more abundantly released from adipose tissue of nonobese than obese subjects (p < 0.05) (A), and omentin was almost exclusively released from the visceral depot (p < 0.001) (B).