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PEL activity of PtxtPL1-27 expressed in Escherichia coli. PEL activity was measured as the formation of unsaturated uronides. Effect of pH (A) and Ca2+ ions (B). Means of five replicates ± standard error. Vector denotes the proteins extracted from the bacteria expressing the vector without PtxtPL1-27. EDTA, ethylenediaminetetraacetic acid.
