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. 2014 Jan 22;7:11. doi: 10.1186/1754-6834-7-11

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Copyright © 2014 Biswal et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Overexpression of PtxtPL1-27 affects extractability of acidic polymers from wood. Wood alcohol-insoluble residues (AIRs) were sequentially extracted with water, 1,2-cyclohexylenedinitrilotetraacetic acid (CDTA) 1 M potassium hydroxide (KOH) and 4 M KOH, and the amounts of uronic acids extracted by each solvent and remaining in the pellet was determined by the biphenyl assay and expressed per weight of extracted cell wall material. Data are means of three biological replicates and standard error. Probability refers to the post analysis-of-variance analysis by contrast between transgenic lines and wild-type (WT).