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YB-1/p18 detection in human plasma. Human plasma was blotted onto a nitrocellulose membrane and YB-1 detected by immunoblotting using a novel monoclonal antibody. Patients with malignant disorders often displayed an additional signal at 18 kDa (“YB-1/p18”), and its intensity was quantified by densitometry. A positive reference sample was run on each blot (T168). Samples with an 18-kD band ≥0.45 were considered to be YB-1/p18 positive.
