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. 2014 Jan 29;7:16. doi: 10.1186/1754-6834-7-16

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Copyright © 2014 Qi et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Construction of recombinant vectors. (A) Recombinant vector T2ΔbudC for budC knock-out. The vector contained a temperature-sensitive replicon from B. subtilis (194-Ori), Kanamycin-resistant gene (Kan), and the homologous arm A and B for homologous recombination. (B) Recombinant vector of pHYbudC for expression of budC in B. licheniformis. The vector contained budC expression cassette including P43 promoter, the budC gene and the α-amylase gene (amyL) transcription terminator of B. licheniformis WX-02.