Table 1.
Bacterial strains and plasmids used in this study
| Strains and plasmids | Characteristics a | Source or reference |
|---|---|---|
|
E. coli strains |
|
|
| DH5α |
F– Φ80d/lacZΔM15, Δ(lacZYA-argF) U169, recA1, endA1, hsdR17 (rK–, mK+), phoA, supE44, λ–, thi-1, gyrA96, relA1 |
Laboratory stock |
|
B. licheniformis strains |
|
|
| WX-02 |
CCTCC M208065, wild type |
Laboratory stock [14] |
| WX-02 ΔbudC |
budC knock-out mutant of WX-02 |
This study |
| WX-02 ΔbudC/pHYbudC |
plasmid-based budC complementation strain of WX-02 ΔbudC by introduction of pHYbudC, Tcr |
This study |
| Plasmids |
|
|
| T2(2)-ori |
E. coli-B. licheniformis shuttle vector, oripUC/orits, temperature-sensitive, Kanr |
Laboratory stock |
| T2ΔbudC |
T2(2)-ori derivative containing homologous arms for budC knock-out |
This study |
| pHY300PLK |
E. coli-B. licheniformis shuttle vector, Apr(E. coli), Tcr(E. coli and B. licheniformis) |
TaKaRa |
| pHYbudC | pHY300PLK derivative containing budC, P43 promoter and TamyL (amyL terminator), Apr(E. coli), Tcr(E. coli and B. licheniformis) | This study |
aTcr, tetracycline resistance; Apr, ampicillin resistance; orits, temperature-sensitive Bacillus origin of replication; Kanr, Kanamycin resistance; CCTCC, China Center for Type Culture Collection.