Table 2.
Primers used in this study
| Primer name | a Sequence 5′ → 3′ |
|---|---|
| ΔbudC-A-F |
CGCGGATCCAAAGCGCATGTTTTAAAAC |
| ΔbudC-A-R |
CCGCCCTCCATATAGAATATAATTTTAAAAATAAACATCTTCTTTCTATAAGTAA |
| ΔbudC-B-F |
ACCAATTACTTATAGAAAGAAGATGTTTATTTTTAAAATTATATTCTATATGGAG |
| ΔbudC-B-R |
GCTCTAGACCTCGCACTAGTGTATTTTGAAAC |
| ΔbudC-F |
CGAACTCCATGAACTGACAGTC |
| ΔbudC-R |
TTGCTATTTCCTGTTATGACC |
| P43-budC-TamyL-1 |
CGCGGATCCTGTCGACGTGCATGCAGG |
| P43-budC-TamyL-2 |
CAATTTTTCCAGATACTTTACTCATGTGTACATTCCTCTCTTACCTATA |
| P43-budC-TamyL-3 |
TATAGGTAAGAGAGGAATGTACACATGAGTAAAGTATCTGGAAAAATTG |
| P43-budC-TamyL-4 |
CGTCCTCTCTGCTCTTCTATCTTTTAATTAAATACCATTCCGCCATC |
| P43-budC-TamyL-5 |
GATGGCGGAATGGTATTTAATTAAAAGATAGAAGAGCAGAGAGGACG |
| P43-budC-TamyL-6 |
CCGGAATTCGATCACCCGCGATACCGTC |
| ΔbudC A signal crossover-F |
CTTCACATGGACGATCCTAAT |
| ΔbudC A single crossover-R |
TGTTCCTCCGTAAACCGCTAAG |
| ΔbudC B single crossover-F |
CAACCACCCCTATTGAAAGCAT |
| ΔbudC B single crossover-R | GATACCTGTCCGCCTTTCTCC |
aRestriction sites highlighted in bold. Italics stands for the overlap region for splicing by overlapping extension PCR (SOE-PCR).