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. 2014 Jan 30;14:4. doi: 10.1186/1471-213X-14-4

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Copyright © 2014 Czarniewska et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Confocal and fluorescent micrographs of control (A, C) and treated (B, D) terminal follicles of T. molitor. In control follicular cells stained with orange acridine, lysosomes are not detected and nuclei of follicular cells (green colour) and oocyte (dark colour) are visible (A). In the follicular cells of treated females orange acridine stain shows many lysosomes (yellow colour, arrow) (B). In isolated control follicular cells stained with MDC, autophagic vacuoles are not detected (C). In isolated follicular cell of treated females stained with MDC arrow shows autophagosome (blue colour) (D). Females were injected with saline (control) or 10 nmole Neb-colloostatin (treated) on day 3 and assayed on day 4.