Fig. 3.

p62 depletion impairs NF-κB activation following IL-1β stimulation. HEK293 cells expressing shRNAs targeting human p62 or a control shRNA (scramble) were cotransfected with pNF-κB – luciferase plasmid and a β-galactosidase reporter vector. 24 h later, cells were treated with the indicated stimuli (TNFα, 20 ng/ml; IL-1β, 20 ng/ml) for 4 h and luciferase activity was determined. Data shown represents relative luciferase activity normalized against β-galactosidase activity and is representative of six independent experiments performed in triplicate.