FIGURE 2.

Neutrophil infiltration and development of stromal haze in TLR4^−/− bone marrow chimeric. Recipient C57BL/6 and TLR4^−/− mice were irradiated and reconstituted with bone marrow from eGFP⁺ C57BL/6 (B6) or eGFP⁺ TLR4^−/− mice. After 2 (A) or 6 wk (B and C), corneas were abraded and stimulated with LPS, Pam₃Cys, or Hanks’ buffered salt solution (HBSS), and neutrophils/5 μm section and stromal haze (reflectivity) were examined after 24 h as described above. A and B, Significant differences (*, p < 0.05; **, p < 0.01) were noted as follows: 1) LPS vs HBSS in B6 → TLR4^−/− chimeras; 2) LPS-treated B6 → TLR4^−/− vs TLR4^{−/ −} → TLR4^−/− chimeras, and 3) LPS-treated B6 → TLR4^−/− vs B6 → B6 chimeras. No differences were detected in Pam₃Cys-treated B6 → TLR4^−/− vs TLR4^−/− → TLR4^−/− chimeras. C, Significant differences are indicated between LPS and HBSS-treated B6 → TLR4^−/− and B6 → B6 chimeras.